Beneficial prophylactic effects of poly(I:C) and zymosan against Schistosoma mansoni infection: MSc Thesis Abstract

MSc Thesis Abstract Background: The use of vaccination in the control schistosomiasis at a population level induced low cure rates and also resistance to drugs has been recently recorded Egyptian patients. For these reasons, search for new with adjuvant anti-schistosomal activity is urgently needed. Aim study: study focused on newly synthesized ligands that play central role as inducers innate adaptive immune responses are therefore considered potential targets vaccine adjuvants. These include Polyinosinic: polycytidylic acid (Poly (I:C) (TLR3L) and  Zymosan (TLR2L) which have displayed an impressive array biological activities including antitumor antiviral effects. present investigated their effects S. mansoni-infected mice. Materials Methods: was divided into two experimental settings treatment settings. In settings, mice were assigned 8 groups. Mice vaccinated 2 times weeks intervals, then infected mansoni. Five post-infection, euthanized analysis. non-vaccination settings,  determination worm burden, tissue eggs count, assessment liver function tests, parameters oxidative stress done. Results: Vaccination by Poly increased counts platelets, segmented neutrophils, eosinophils, monocytes while decreases lymphocytes indicating immunological effect ligands. current showed soluble antigen protein (SWAP) associated improvement enzymes compared SWAP alone. apparent granuloma volume. TLR had no side normal healthy reduced adverse caused mansoni through enzymes, stress, reduction ova count.  Conclusion: This first assesses adjuvants infection. Our recommendation increase time both significant preserve parameters. (This thesis approved Faculty Science, Tanta University, Egypt November 26, 2012).